مقالات  دكتر ماريا ظهيري  


  The effect of Matrigel on the developmental process of mouse blastocysts • Journal of Reproduction and Infertility. 2006 Zahiri M, Ghafari M., Niknafs B,Heidari M. اول از چهارم
Introduction: Culturing embryos on Matrigel™, is one of the most suitable methods for study-ing in vitro embryonic developments. As Matrigel has not been used extensively in different species for embryonic development studies, this study was undertaken to determine the effects of Matrigel on the developmental processes of mouse blastocysts. Materials & Methods: To a number of female NMRI mice, hMG and HCG injections were made for ovulatory stimulation and then they mated with males from the same strain. Later on, blastocysts were obtained and randomly divided into 2 groups: 150 case blastocysts and 134 control blastocysts. Blastocysts were cultured for 48 hours in M16 medium, supplemented with 4mg/ml of bovine serum albumin (BSA). Later, the blastocysts were compared with the blastocysts cultured in Matrigel plus the same medium. Developmental studies were carried out every 24 hours for 2 days. The data was analyzed by SPSS software and the results were tested by chi-squared. Results: After 24 hours, a significantly higher ratio of embryos reached the hatched blastocysts stage Ι in the case group (74%), compared with that of the control group (52.2%), (p<0.05). At the same time the percentage of fragmented blastocysts in the control group was 11.9% which was significantly higher than the case group (2%), (p<0.05). After 48 hours, 41% of blastocysts cultured in the control medium, developed to stage I, the value being significantly more than the blastocysts in the case group (p<0.05). Moreover, after the same period of time (48 hours), the percentage of stage II hatched blastocysts in the case group (79%) was higher than the control groups (59%), (p<0.05). Conclusion: Matrigel use in enriched culture media can increase development and growth of mouse blastocysts. It also seems that ultrastructural studies of cultured embryos or immunocyto-chemical studies from this regard would be beneficial in understanding the processes involved.

  Successful Transplantation of Frozen-thawed Calf Spermatogonial Stem Journal of Isfahan Medical School 2013 Rahimi-Feyli, Peyman; Tajik, Parviz; Koruji, Morteza; Shafiei, Shiva; Zahiri, Maria پنجم از 5
Background: Spermatogonial stem cells (SSCs), the only adult stem cells capable of transferring genetic information to the next generation, are important in cell biology. The most important applications of these cells include the treatment of infertility, fertility preservation of oncological patients, and the creation of transgenic animals. The aim of this study was to evaluate the possibility of transplantation of frozen-thawed bovine spermatogonial cells to the testis of an azoospermic mouse model and to prove the presence of spermatogonial stem cells among bovine spermatogonial cells isolated and proliferated by methods used in the study. Methods: Spermatogonial cells were isolated from 3 to 5 month-old bull calves and then were frozenthawed. Subsequently, the cells were cultured in the presence of glial cell line-derived neurotrophic factor (GDNF 40 ng/ml) for 2 weeks. The stemness of these cells was evaluated by transplanting them to the testis of azoospermic mouse models. Findings: The viability rate of fresh cells and frozen cells after thawing were 87.4 ± 2.4 and 65 ± 1.7, respectively, that corresponds to a significant difference (P < 0.01). After one month, the mice were euthanized and the histopathological evaluation of their testes with fluorescent microscope showed the colonization of marked bovine SSCs in the mouse seminiferous tubules. Conclusion: In our knowledge, this is the first reported case of xenotransplantation in Iran. The results of this study showed that the xenogeneic transplantation of calf frozen-thawed spermatogonial cells to the mouse testis has been successful and SSCs were present among the transplanted cells.

  Expression profile of germ stem cell-specific genes in human spermatogonial stem cells after co culture with sertoli cells ISMJ 2014, 17(2): 150-160 Maria Zahiri1, Mansooreh Movahedin *2, Seyyed Javad Mowla3, Mehrdad Noruzinia4, Mohammad Reza Noroozi5 , Naser Amirjanati6 اول از 6
Background: Human spermatogonial stem cells (SSCs), are the foundation of spermatogenesis. Because of low number and lack of significant marker in human SSCs, studying their characteristics, could provide better understanding about the biology of male fertility. This study was designed to examine the effects of in vitro co-culture with sertoli cells on SSC colonization and germ cells specific gene expression of human spermatogonial stem cells. Material and Methods: Testicular cells were isolated from testis biopsies by using two step enzymatic digestion and differential plating. two culture system were designed: co-culture with patient Sertoli cells and culture of SSC without co-culture(as control group). The number and diameter of colonies were evaluated during 3 weeks of culture. The expression of alpha 6 integrin, beta1 integrin and PLZF, as germ stem cell specific markers, was assessed using quantitative RT-PCR. Statistical analysis was performed using one way ANOVA in SPSS vesion 16 software with 95% Confidence interval . Result: Our results were showed that the number and diameter of colonies increased significantly in co-culture with sertoli cells (P<0.05). The expression profile of genes in 2nd and 3rd weeks of culture revealed that there is significant higher expression of germ stem cell markers in our co-culture group versus control group. Conclusion: Based on the optimal effects of sertoli cells on spermatogonial stem cells, co culture of the human SSCs with the feeder layer sertoli may be used as a suitable method for the enrichment of human spermatogonial stem cells.

  . Comparing the efficiency of different culture systems on proliferation and purification of the human spermatogonial stem cells. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE 9(1)2011 ISI 0.275 ZAHIRI M.*, MOVAHEDIN M., MOWLA S., NORUZINIA M. اول از 4
Introduction: The aim of this study was to providing an appropriate in vitro system to proliferate and enrichment of human spermatogonial stem cells from obstructive asospermic patients. Materials and Methods: Testicular cells isolated from testis biopsies after enzymatic digestion. Four cultural systems were disigned: co-culture with sertoli, culture on gelatin coated dishes, culture of testicular fragments and culture on uncoated dishes. The rate of colony formation and the diameter of each colony were investigated. Results: Our results showed that there are significant differences (p<0.05) in colony numbers and the time of colony formation in the culture of testis fragments compared with other groups. Culture of testis fragmenteds showed the more number of colony and colonies appeared more rapidly. Regarding the diameter of colonies, there are not any significant differences between the three groups, while culture of isolated spermatogonial cells on uncoated dishes showed the lowest colony diameter between groups. Conclusion: In conclusion designing appreciated cultural system is very important in culture, proliferation and enrichment of SSCs and it should be considered to close these systems to in vivo.

  COMPARING THE EFFICIENCY OF DIFFERENT CULTURE SYSTEMS ON PROLIFERATION AND PURIFICATION OF THE HUMAN SPERMATOGONIAL STEM CELLS INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE 2012 ISI 0.275 ZAHIRI M.*, MOVAHEDIN M., MOWLA S., NORUZINIA M. اول از 4
Introduction: The aim of this study was to providing an appropriate in vitro system to proliferate and enrichment of human spermatogonial stem cells from obstructive asospermic patients. Materials and Methods: Testicular cells isolated from testis biopsies after enzymatic digestion. Four cultural systems were disigned: co-culture with sertoli, culture on gelatin coated dishes, culture of testicular fragments and culture on uncoated dishes. The rate of colony formation and the diameter of each colony were investigated. Results: Our results showed that there are significant differences (p<0.05) in colony numbers and the time of colony formation in the culture of testis fragments compared with other groups. Culture of testis fragmenteds showed the more number of colony and colonies appeared more rapidly. Regarding the diameter of colonies, there are not any significant differences between the three groups, while culture of isolated spermatogonial cells on uncoated dishes showed the lowest colony diameter between groups. Conclusion: In conclusion designing appreciated cultural system is very important in culture, proliferation and enrichment of SSCs and it should be considered to close these systems to in vivo.

  The effect of nanofiber matrix on expression profile of human spermatogonial stem cell gene from obstructive azoospermic patients Reproductive Biomedicine Online Journal 2012;24 ISI 2.796 Maria Zahiri, Mansoureh Movahedin, Seyed Javad Mowla, Mehrdad Noruzinia اول از 4
Introduction: Spermatogenesis is a highly organized process of Spermatogonial stem cells (SSCs) proliferation and differentiation that aretightly regulated by the interaction between the SSCs and their surrounding microenvironment. It seems that the fate of the SSCs has been promoted by the chemical and physical properties of the Extracellular Matrix (ECM) as their surrounding microenvironment. Along this issue, effect of nanofiber matrix as ECM on expression profile of human Spermatogonial Stem Cell genes was investigated. Method: Testicular cells isolated from testis biopsies of non obstructive azoospermic patients by two step enzymatic digestion and preplating method. Spermatogonial cells were cultured either on a nanofiber (as our sample group) or a non-nanofiber surface (as control group) for 3 weeks. The rates of colony formation were investigated every 5 days. Some SSCs markers such as PLZF and GFR-a6 were confirmed using immunofluorecent staining and the expressions profile of some genes such as a6 and b1 integrin, PLZF, c-myc, oct-4 and nanog were determined by real-time PCR at the end of the 2nd and 3rd weeks of culture. Results: Our results showed that culture on nanofiber significantly enhanced the proliferation of SSCs and colony numbers compared with the growth of SSCs on the non-nanofiber surface. The results of RT-PCR between these groups revealed the higher expression of germ stem cell markers in culture on nanofiber surface versus control group. Our data showed the higher significant expression of a6 and b1 integrin on 2nd week of culture in our sample group (Prdweek, expression of a6 integrin, nanog and c-myc were significantly decreased while such markers as b1 integrin and PLZF showed higher expression levels. Conclusion: It is concluded that a nanofiber surface can provide a suitable microenvironment for in vitro culture of SSCs and promotes proliferation and characteristics of human SSCs

  Co-culture of human spermatogonial stem cells with normal sertoli cells can promote characteristics of human spermatogonial stem cells obtained from obstructive patients Human Reproduction. 2012 ISI 4.635 ZAHIRI M.*, MOVAHEDIN M., MOWLA S., NORUZINIA M. اول از 4
Introduction: It has been established that only the pool of mature spermatozoa that have completed their maturation process through the transit in the male genital tract sustain the adequate competence. This is to reach the oocyte, penetrate through the cumulus-corona cells, binds to ZP proteins, undergo acrosome reaction, fuse with the oolemma, and finally deliver, at the time of syngamy, an integer paternal genome. It has been proposed that spermatozoa with these properties display on their membrane hyaluronic acid (HA) receptors. Furthermore, the HA selection would identify spermatozoa that are chromosomally normal and that contain an integer DNA capable of supporting embryo development. In this work we carried out the selection of spermatozoa that exhibit HA binding sites on which we assessed chromosomal status and chromatinic competence. Materials and Methods: Samples from known fertile donors and men undergoing male infertility screening were included. Semen characteristics were noted and thereafter, highly motile spermatozoa were selected by density gradient. Spermatozoa displaying the HA-r were selected in PICSI® dishes. HA-bound and HA-unbound sperm cells were picked up individually by an ICSI pipette and assessed by Diff-Quik™, Aniline Blue, SCD, TUNEL, and FISH. The threshold utilized for each assay was ≥4% normal forms, 80% chromatin condensed, ≤18% DNA fragmentation, ≤15% DNA fragmentation, and 98.4% chromosomally normal, respectively. Assessments were also carried out on the initial samples and on the enriched motile fractions as reference. Results: A total of five specimens were used with an average male age of 31.5 ± 2 years and semen parameters with 33.0 ± 10 x 106/ml density, 46.0 ± 26% motility, and 5.0 ± 3% normal morphology. Male gamete characteristics according to the expression of HA binding sites are given in the Table. View this table: In this window In a new window

  Comparing the efficiency of different culture systems on proliferation and purification of the human spermatogonial stem cells from obstructive azoospermic patients International Journal of Reproductive BioMedicine 2013 0.19 ZAHIRI M.*, MOVAHEDIN M., MOWLA S., NORUZINIA M. اول از 4

  • Combination of coated nanofiber matrix and cell sorting on purification of human spermatogonial stem cell nternational Journal of Reproductive BioMedicine 2013 ISI 0.19 ZAHIRI M.*, MOVAHEDIN M., MOWLA S., NORUZINIA M. اول از 4

  Co-culture of human spermatogonial stem cell can promote characteristics of human spermatogonial stem cell obtained from obstructive azoospermic patients. Human Reproduction 2012 ISI 2.796 ZAHIRI M.*, MOVAHEDIN M., MOWLA S., NORUZINIA M. اول از 4

  Could Co-Culture with Sertoli Cells Effect on Characteristics of Human Spermatogonial Stem Cells? Yakhteh 2012 ISI 1.339 ZAHIRI M.*, MOVAHEDIN M., MOULA S.J., NORUZINIA M. اول از 4
Objective: Along the biological understanding of spermatogenesis, a major concern is improving the efficiency of the various cultural conditions and evaluation of the safety of the clinical application. Since the interaction between SSCs and their nich is capable of inducing self-renewal in SSCs, suggesting that this signaling pathways are promoted in culture of SSCs, by co-culture with normal sertoli obtained from healthy persons Materials and Methods: Testicular cells isolated from human testis biopsies by two step enzymatic digestion and plating methods. The identity cells were confirmed through immunocytochemistry. We were designed various culture system: co-culture with patient own Sertoli cells, co-culture with normal sertoli cells obtained from a person with normal spermatogenesis and culture of SSC on un-coated dishes. Colonization was evaluated during the 3 weeks of culture. The expression of the a6 and b1 integrins and PLZF as germ stem cell specific markers and nanog, c-myc and oct-4 as pluripotency markers were assessed using quantitative RT-PCR on 2nd and 3rd weeks of culture. Results: Our result showed higher mean in number and diameter of colonies in co-culture groups in the compare with control group. Our data was shown the higher expression of germ stem cell markers during the culture and lower expression of nanog in 3rd weeks in co-culture with patient own Sertoli cells. Co-culture with normal sertoli cells in revealed significant higher expression of PLZF. At 3rd weeks, there are significantly increased in expression profile of a6 and b1 integrins and PLZF in compare to our control group. Co-culture with normal sertoli showed higher expression of germ stem cell specific markers at 3rd weeks of culture versus co-culture of SSCs with the patient own sertoli. Conclusion: Co-culture of human SSCs with sertoli cells has emerged as a suitable method for the enrichment of spermatogonial germ cells.

  • Isolation and Purification of Human Spermatogonial Stem Cells Using Different Cultural Systems Yakhteh 2012 ISI 1.339 ZAHIRI M.*, MOVAHEDIN M., MOWLA S., NORUZINIA M. اول از 4
Objective: The fate of spermatogonial stem cells, as the foundation of spermatogenesis are tightly regulated by some intrinsic factor and extrinsic factors from their nich. Along the biological understanding of these mechanisms, a major concern is improving the efficiency, evaluation of the safety of their clinical application. Because the mechanisms that involved in human spermatogenesis are complex and unknown, this study was designed to providing an appropriatein vitro system to proliferate and enrichment of hSSC. Materials and Methods: The isolation of spermatogonial stem cells (SSCs) were performed using two step enzymatic digestions and plating method. The identity of the SSCs and sertoli cells was confirmed through immunocytochemistry. We were designed 4 various culture system: co-culture with patient own Sertoli cells, co-culture with normal sertoli cells obtained from a person with normal spermatogenesis and culture of SSC on un-coated dishes and culture of testis cells suspension. The number and diameter of colonies were evaluated during the 3 weeks of culture. The identity of the SSC and sertoli cells was confirmed by immunocytochemistry against PLZF, GFR-a1and vimentin. The expression profile of the several germ stem cell specific and pluripotency markers were assessed using quantitative RT-PCR. Results: Significant differences were observed between the four groups (p<0.05), with higher mean in number and diameter of colonies for co-culture with testis cells suspension in compare with other groups (p<0.05). Analysis of marker expression revealed that there are higher expression of germ stem cell markers and lower expression of pluripotency markers in co-culture with Sertoli cells. Conclusion: Our findings demonstrated that adult coculturing of SSCs with Sertoli cells can influence spermatogonial proliferationin vitro.

  • Affirmation of Embryo Transfer at Blastocyst stage in assisted reproductive system Yakhteh 2007 ISI 1.339 Zahiri M, Ghafari M., Niknafs B,Heidari M. اول از 4

  • The efficiency of magnetic-activated cell sorting inn the purification of human spermatogonial stem cell from obstructive azoospernic patient. 10th Iranian Anatomical Sciences Congress abstract book 2012 ZAHIRI M.*, MOVAHEDIN M., MOWLA S., NORUZINIA M. اول از 4

  • The effect of human spermatogonial stem cell niche on their enrichment and colony formation Iranian Congress on biology and applications of stem cells 2011 ZAHIRI M.*, MOVAHEDIN M., MOWLA S., NORUZINIA M. اول از 4

  • Affirmation of Embryo Transfer at Blastocyst stage in assisted reproductive system Yakhteh 2007 1.339 Zahiri M, Ghafari M., Niknafs B,Heidari M. اول از 4

  • Applicable progress of simulation sciences, Yes or No! 2nd International Congress of Medical Ethics in Iran Abstract book. (2008 Thran-Iran) 2008 اول از 3

  In vitro preparation of animal model to investigate the implantation stages • 10th International Congress of Obstetrics and Gynecology Abstract book 2006 اول از 3

  • Tissue engineering in ART services • 7th International Congress of Obstetrics and Gynecology diseases 2006 اول از 3

  • comparing the proliferation and purification of human spermatogonial stem cells in different culture systems Abstract book middle east fertility society 2013 ZAHIRI M.*, MOVAHEDIN M., MOWLA S., NORUZINIA M. اول از 4

  • پيشرفت كابردي علم شبه سازي و بايد ها ونبايدها دومين كنگره بين المللي اخلاق پرشكي ايران 1387 اول از 3

  • طب اسلامي الگوي بي بديل ومنطقي جهت ارتقا كيفيت زندگي امروز همايش مديريت طب اسلامي 1386 اول از 3

  كاهش جنين در حاملگي هاي چند قلويي مصالح پزشكي وحقوق والدين سمينار اخلاق پرشكي وحقوق باروري با محوريت سقط درماني وكاهش جنين 1391 دوم از 2

  • سقط درماني از ديدگاه مكاتب فلسفه اخلاق سمينار اخلاق پرشكي وحقوق باروري با محوريت سقط درماني وكاهش جنين 1391 دوم از 2

  • Stem cells in review Iranian South Medical Journal 2014 hاول از سوم
In recent years, many progresses have been made in the field of the stem cell researches that is a promising novel therapeutic strategy for the incurable disease. These cells exist in all multicellular organisms, having an ability to divide and differentiate into a diverse range of specialized cell types and they also can replace the lost and damaged cells. Stem cell’s property of self-renewal and their potency have been proposed a promising usage of these cells in the future in regenerative medicine, cell therapy and drug researches. Recent technologies provide an unlimited source of autologous and non-autologous stem cells. Stem cell therapy has some restrictions so further research to improve our biological understanding is essential. In present paper, basic concepts, applications, limitations and the prospect of using stem cells for future use have been reviewed.

  • Human spermatogonial stem cell tracking ex vivo IJRM 2014 ISI 0.19 ZAHIRI M.*, MOVAHEDIN M., MOWLA S., NORUZINIA M. اول از 4

  • Concepts, consequences and implications of noninvasive imaging in stem cell studies 2014 ZAHIRI M.*,, Asadi M.. اول از دو

  Attitudes of medical students towards effectiveness of Peer Assisted Learning system in anatomy 1395

  تاثیر مواجهه زودرس با محیط بالینی در انگیزه و دیدگاه آنها نسبت به درس آناتومی در دانشجویان سال اول پزشکی 1395

  Marine Invertebrate’s Stem Cell Culture: Biotechnology Prospects of Marine Stem Cells Iran South Med J 2016, 19(5): 912-930 Reyhane Zahiri1, Mariya Zahiri
Since marine invertebrate have many applications in medicine and biological sciences and pharmaceuticals because of valuable metabolites, in recent years related studies to marine invertebrate’s stem cells culture have dramatically increased. Stem cells Stem cells are considered as the progenitor cells of the body. In fact, culturing of these cells is a developed cell culture that maintains ability to proliferate throughout adult life from birth to death and also these stem cells are able to differentiate into different cell types. Unfortunately related researches to stem cells in marine invertebrates didn’t develop in the same manner of mammals and despite many efforts; mana cell lines have been not derived from these organisms yet. This would be due to the paucity of available resources as well as lack of maintaining of culture conditions that this is mainly due to lack of sufficient knowledge about the mechanisms and systems involved in maintaining their stemness. Many evidences have been reported about the presence of marine invertebrates’ stem cells in hydrozoans, crustaceans, echinoderms and real chordate so far. Understanding the suitable culture conditions and understanding the needs and providing appropriate cellular microenvironment will be the prospect of using this valuable resource. This systematic review will be present an overview of the concepts and activities related to marine invertebrates stem cells.

12

< >